FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1716355
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28630.16680698340378303No Hit
GTGTTAGCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC28230.16447646320254258No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20880.1216531545047499No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19610.11425375286581156No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17730.1033003079199816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23800.040.5572661
GTATCAA58400.040.1153761
TATCAAC68600.034.097642
TCAACGC72250.032.7087974
AACGCAG72950.032.6303676
ATCAACG72650.032.481433
CAACGCA73000.032.4198045
TGGTATC9800.031.1935842
GTGGTAT10050.031.1038861
AGAGTAC78150.029.92826511
CGCAGAG80500.029.2687578
CAGAGTA79800.029.26640910
ACGCAGA81450.029.054737
GCAGAGT81700.028.7127469
CTTATAC3600.025.7632451
TGTTAGA7650.025.28172370
TCGTATG1401.3251338E-824.75206240
TTAACGG2800.024.64701735
AGTACGG63300.024.63433813
CGTATGC1701.5643309E-1024.4795741
Produced by FastQC (version 0.11.5)