FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369060
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCCA150.00237798268.825884
AATTTTG200.00424020659.47716569
CTCTTAT200.00742098151.6334041
TCTTATA200.007432920451.6124152
AGAGACA405.0704315E-543.0161746
GGTATCA4750.042.03141
GTATCAA11900.040.786051
TATCAAC13450.036.0711332
CTTATAC501.8837793E-434.4222681
GTGGTAT1500.034.4222681
TACACAT501.8867827E-434.4129375
AGAGTCG400.002628146434.4082811
TGGTATC1600.032.257762
GACAGTG553.3013272E-431.2844927
AACGCAG15750.031.2447626
CAGAGTA15750.030.58513610
CAACGCA16100.030.5655295
GCAGAGT16300.030.4016159
AGAGTAC16000.030.10724311
TTATACA703.9108534E-529.4968054
Produced by FastQC (version 0.11.5)