FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369060
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6210.16826532271175418No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGATA200.00680881252.7697162
TCAAAAC200.007004956252.39216252
GTGGTAT1850.048.3431851
TATAAGA451.8962019E-645.8640442
ATAAGAG451.8979717E-645.8578343
TAAGAGA451.8979717E-645.8578344
TGGTATC1900.045.260572
GTATAAG503.914525E-641.277641
GGTATCA5100.039.7938041
GTATCAA12850.039.350281
GGCTTAC350.00136636639.3120421
TAGGAAG350.001367276539.3067135
TATCAAC13750.036.5244562
TGTAGGA501.8915918E-434.3980332
AGAGTCG501.893099E-434.393375
CAGAGTA15950.031.26670310
AGAGTAC16100.031.18902211
GCAGAGT16150.030.8795019
AACGCAG16300.030.8063376
CAACGCA16300.030.5953355
Produced by FastQC (version 0.11.5)