FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1261674
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACAGACTCCTCGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA72900.5778037749846633No Hit
GTCTGTAGACCTTGCATGCAGTAATAAATTCCCACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAGT55010.43600803377100583No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47370.3754535640743964No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47020.3726794718762533No Hit
GTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGCTT38410.304436803801933No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36980.29310265567809113No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32000.25363128668736934No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30130.23880970837157617No Hit
ATCCATTACTATGCAAGAGGCTCTGACTAGACCTACAGGAGATGTCGGCC29490.2337370826378288No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27390.2170925294489702No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27000.21400139814246785No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26820.21257472215485143No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26440.20956285062543892No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26270.20821543441491225No Hit
GTGTAAAGTCTGTGCCGGATCCACTGCCAGTGAACCTGTCAGGGACCCCG25070.19870426116413592No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24980.1979909231703277No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTC24140.19133310189478422No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23070.1828523057461753No Hit
GTCCCTGACAGGTTCACTGGCAGTGGATCCGGCACAGACTTTACACTGAA21110.16731738943657395No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20890.1655736743405983No Hit
GACTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTGGGAATTTATTACTGCATGCAAGGTCTACAGACT20840.16517737545514927No Hit
GTCAGGGACCCCGGAGGCCCGATAAGAAGCCAAATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTA20530.16272032236536538No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19670.15590398153564233No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19210.15225803178951142No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18600.14742318538703342No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGATCCGGCCGACATCTCCT18440.14615502895359658No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG18150.14385649541799228No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT17900.1418750009907472No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGCTTCTTATCGGGCC17510.1387838696842449No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17290.13704015458826924No Hit
CCCCTGGAGATCCGGCCGACATCTCCTGTAGGTCTAGTCAGAGCCTCTTG16720.13252234729415047No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16460.1304615930898156No Hit
GGTCTACAGACTCCTCGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT16120.1277667606687623No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTCCGAGGAGT15660.12412081092263137No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15640.12396229136845174No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15570.12340747292882313No Hit
CTCCACAACTCCTGATCTATTTGGCTTCTTATCGGGCCTCCGGGGTCCCT15550.12324895337464353No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15430.1222978360495659No Hit
CTGTAGACCTTGCATGCAGTAATAAATTCCCACATCCTCAGCCTCCACTC15090.1196030036285126No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14850.11770076897835732No Hit
CTCCCTGCCCGTCACCCCTGGAGATCCGGCCGACATCTCCTGTAGGTCTA14480.11476815722603462No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14260.11302444213005895No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13850.10977479126937705No Hit
GGTCTAGTCAGAGCCTCTTGCATAGTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC13700.10858589461302999No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC13640.10811033595049116No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGGTGTATCCATTACTATGC13590.10771403706504216No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT13400.10620810130033591No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13240.10493994486689906No Hit
GTTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAGGTGTATCCATTACTATGCAAGAGGCTCTGA12970.10279993088547437No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA12840.10176955378330695No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC12830.10169029400621714No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12720.1008184364582293No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12650.10026361801860069No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCGA350.00509903256.77624570
GGTATCA11200.039.121971
GTATCAA27700.037.842171
TATCAAC30600.034.356432
ATCAACG31050.033.6388053
TCAACGC31300.033.5854154
CGTCTAG450.00311505433.24152866
CTGTCTT30500.032.57653470
AACGCAG32500.032.2416656
CAACGCA32550.032.088635
ACGCAGA35500.029.611937
AGAGTAC35750.029.40601711
CAGAGTA36000.029.2018110
CGCAGAG36900.028.4884418
TATATCG606.2276726E-428.077555
AGTACGG30250.026.95551513
TGTTAGA14850.026.76321670
GAGTACG32050.025.65189612
CGACCGG2351.03016646E-725.3681170
GTGGTAT5200.025.278811
Produced by FastQC (version 0.11.5)