FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences987564
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32480.3288900769975414No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29810.3018538545349972No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28170.2852473358688652No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26930.2726911876091068No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26180.26509674309715625No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC24460.2476801503497495No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22970.2325925205860076No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22220.22499807607405697No Hit
GTAATACATAGCCGTGTCTGAGGCGGTCACAGAGCTCAGCCTCAGGGAGA21310.21578348339955689No Hit
CAGTAATACATAGCCGTGTCTGAGGCGGTCACAGAGCTCAGCCTCAGGGA20550.20808777962744693No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20420.20677140924537546No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20380.20636637220473814No Hit
ATGTATTACTGTGTGGGCCCTAGAGACCCTTCGGTGGTACGGTCTCTGATCAAGTTGGGTGCTCCTTTTGATAT20050.20302481661947985No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20040.2029235573593205No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19980.20231600179836445No Hit
GTATTACTGTGTGGGCCCTAGAGACCCTTCGGTGGTACGGTCTCTGATCA18830.19067118688004017No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18560.18793718685573796No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC17650.17872259418123787No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17600.1782162978804412No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16980.171938223750562No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16570.16778659408402896No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16520.16728029778323228No Hit
ACCTACTACTCCCCGTCCCTCAAGAGTCGAGTCACCGTTTCCGTAGACACGTCCAAGAACCAGTTCTCCCTGAGG16110.16312866811669927No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16060.16262237181590258No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15980.16181229773462785No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTACTTACTTCTGGGGCTGGATCCG15870.16069844587287507No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14970.15158511245853432No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG14850.15037000133662223No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14440.14621837167008925No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14220.14399066794658372No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA14110.14287681608483097No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13900.1407503716214848No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13680.1385226678979793No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13120.13285214932905615No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA13110.13275089006889682No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12890.13052318634539128No Hit
GTAGTAGGTGGTCCCACTATGATAGATACTCCCAATCCACTCCAGCCCCTTCCCGGGGGGCTGGCGGATCCAGCC12740.12900429744300115No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC12710.12870051966252313No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12370.12525770481710552No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG12120.12272622331312198No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11840.11989096402866042No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA11800.11948592698802304No Hit
GTAATATGTCGCAACATCTTCAGGCTGCAGGCCGCTGATGGTGAAGGTAAAATCTGTCCCAGATCCACTTGCA11490.11634688992308348No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11470.11614437140276479No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG11430.11573933436212741No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11290.11432170471989662No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11240.11381540841909991No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11180.11320785285814387No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11180.11320785285814387No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT11040.11179022321591309No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10780.1091574824517702No Hit
GTGCAAGTGGATCTGGGACAGATTTTACCTTCACCATCAGCGGCCTGCAGCCTGAAGATGTTGCGACATATTA10640.10773985280953943No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA10420.10551214908603392No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10240.10368948240316576No Hit
CACTTGCACTGAACCTTGGTGGGACCCCCGTCTCCAAATTGGAGGCATCGGAGATCAGGAGCTTAGGGACTTTCC10170.10298066758205038No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10150.10277814906173169No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10150.10277814906173169No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT250.00159992976.0615370
GGTATCA5450.044.1487051
TTAATCG459.921196E-538.3943837
GCTTATC557.582901E-637.4977341
TATACAC1700.036.394863
GTATCAA11300.036.1980321
GTTTGCG901.6491939E-435.21367370
TCGTATG1600.034.62722440
GTGGTAT2000.034.3729251
TGGTATC1900.034.3729252
GTGTAAG400.00264356334.372921
ACGAGAC1600.034.36770222
CGTATGC1550.033.52732541
CTTATAC1450.033.187651
ATACACA2000.032.6542784
TATCTCG1400.032.07366636
ACCGGAG1850.031.60994527
TACACAT1850.031.585935
GTATGCC1650.031.50823242
TATCTGG10650.030.94835570
Produced by FastQC (version 0.11.5)