FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1049211
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC950.054.247671
TTATACA1100.046.8457952
TATACAC1250.041.22433
GTATCAA28400.040.5265731
GACTGTT350.001263372539.9505352
TATCAAC31400.036.760522
GGTATCA11800.036.6861341
TACACAT1700.034.353585
CTCGTAT1600.032.43216739
GTGGTAT4300.031.9598671
ATACACA1750.031.4089894
TGGTATC4600.031.3663142
AGAGTAC36000.031.20004311
GAATAGT450.004262887431.17848456
CAGAGTA36450.031.09756310
AACGCAG37750.031.0319796
TTTACTT450.00451105430.82002442
TCGTATG1800.030.77413240
GCAGAGT37800.030.623029
CAACGCA39050.029.8229525
Produced by FastQC (version 0.11.5)