FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1049211
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14840.14143961510125228No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACA200.003967029560.4947569
GGATCTA200.006368037353.677367
TAATGCT200.007499382451.502994
TTATTGG400.00852957149.84957570
GTGGTAT5850.043.443071
TGGTATC6200.040.9867322
GTATCAA32000.038.7437741
GAATAGT951.8371793E-1036.91113756
TATCAAC34750.035.773142
GGTATCA13700.033.8421441
TGTATAA1056.7666406E-1032.704992
AGAGTAC38650.031.27493911
CAGAGTA38550.031.17790410
AACGCAG40400.030.9402146
GTCTCTA450.00472645530.5275631
CGTTAAC450.00472645530.52756316
GTTACAC450.004731967630.5202883
GCAGAGT40550.030.4023289
GTGTATA1151.7880666E-929.863921
ACGCAGA42700.029.2736457
Produced by FastQC (version 0.11.5)