FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2378894
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62100.039.1988451
GGTATCA26200.038.6359441
TATCAAC71900.033.8982122
GTGGTAT10050.033.2315941
ATCAACG73700.033.0235943
CAACGCA75450.032.485775
TCAACGC75650.032.2178654
AACGCAG76750.031.9803776
AGAGTAC81750.029.55683111
ACGCAGA84050.029.2816187
TGGTATC11350.029.1170772
CGCAGAG84400.029.0786258
CAGAGTA84300.028.98940510
GCAGAGT94400.026.1430179
AGTACGG67750.025.20309813
GAGTACG68650.024.97035412
TCGTATG2300.024.11299140
CGTATGC2700.023.12345341
CGTCTAA1755.504262E-921.91397550
GTATTAA2900.021.3707621
Produced by FastQC (version 0.11.5)