FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1136190
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC6500.043.7008442
TATACAC2300.041.6652873
GGTATCA23050.040.6858021
GTATCAA53850.039.6608621
CTCGTAT1950.033.73501239
ATACACA2900.033.044884
GTGGTAT8400.033.0041851
TACACAT3350.031.6709485
AACGCAG69000.030.9513536
CAACGCA69850.030.7217045
TCAACGC71250.030.0700154
CGTCTAG1900.029.98096363
AGAGTAC72600.029.13115911
CAGAGTA73200.028.9391310
GCAGAGT73800.028.7051189
ATCAACG75600.028.2492523
CTTATAC3300.028.003551
TCGTATG2850.027.97356840
CGCAGAG75750.027.9674058
ACGCAGA76650.027.8176277
Produced by FastQC (version 0.11.5)