FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1136190
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29590.2604317939781199No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC9350.042.792882
GTATCAA58950.040.2687841
GTGGTAT10950.036.8604621
GGTATCA24550.036.3648571
AACGCAG73200.032.4748736
CAACGCA74150.031.9651435
TCAACGC76250.031.1269074
CAGAGTA77550.030.65326310
AGAGTAC77350.030.64408511
GCAGAGT78400.030.27739
CGCAGAG80550.029.4691528
ACGCAGA81450.029.31157
ATCAACG81550.029.1026653
TATCAAC82900.029.0000062
GTAGTTT3450.027.02869670
GAGTACG80600.025.58803212
AGTACGG81700.025.20165413
TCGGTTA1202.6398939E-622.81153723
ATAAGAG1653.4797267E-922.7984853
GTACGGG88700.022.40107714
Produced by FastQC (version 0.11.5)