FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1323644
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT77630.5864870010365325No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC52940.39995648376753873No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG49420.37336323059674653No Hit
ATGCTGCACCCGGTCTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCA47320.35749793751189896No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC46340.35009413407230344No Hit
GATCTATGCTGCACCCGGTCTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA44860.3389128798982204No Hit
GGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATGCTGCACC42770.32312313582806257No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41650.3146616461828105No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC41600.3142839011093618No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT40950.3093732151545279No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG40270.30423588215562497No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT37780.2854241774978771No Hit
GTGCAGCCACAGTTCGTAGGATCTCAACCGTGGTCCCTCCGCCGAAAGTG36870.2785492171611098No Hit
GAATTCACTCTCACCATCAGCAGCCTACAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGGTCAGAGTTCC34040.2571688460039104No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC33790.25528012063666666No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC32620.24644088591796584No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG30390.2295934556421515No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30280.22876241648056425No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAA29900.22589155392235377No Hit
GGGTCAGAGTTCCCCGCTCACTTTCGGCGGAGGGACCACGGTTGAGATCCTACGAACTGTGGCTGCACCAT29160.2203009268353122No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG28970.21886549555620696No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG28280.21365261354261417No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT27420.20715539827929563No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27410.2070798492646059No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26770.20224471232446187No Hit
GCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC26740.20201806528039262No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26310.19876945764873336No Hit
GCCTACAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGGTCAGAGTTCCCCGCTCACTTTCGGCGGAGGGA26030.19665408523742034No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25150.19000577194472232No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24710.18668161529837327No Hit
CCTACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCT22990.1736871847717362No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT22180.16756771458186642No Hit
ATTGTCAACAGGGTCAGAGTTCCCCGCTCACTTTCGGCGGAGGGACCACGGTTGAGATCCTACGAACTGTGGCTG21900.1654523421705534No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA21840.1649990480824149No Hit
CTCTGACCCTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGTAGGCTGCTGATGGTGAGAGTGAATTCTGTC21740.1642435579355174No Hit
GTGCAGCATAGATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAACCAGCTGCTAATACC21640.1634880677886199No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21500.1624303815829634No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA21440.1619770874948249No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTAGGATCTCAACCGTGGTCCCTCCGCCGAAAGTGAGCGGGGAACT21430.16190153848013514No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21000.15865293084847587No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20730.1566131074518526No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA20660.15608426434902434No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20410.15419553898178062No Hit
CACCATCAGCAGCCTACAGCCTGAAGATTTTGCAACTTACTATTGTCAACAGGGTCAGAGTTCCCCGCTCACTTT19940.15064473529136235No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19890.15026699021791357No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC18550.14014342224948703No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18350.13863244195569202No Hit
GTTGAGATCCTACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCC18270.13802804983817402No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17870.135006089250584No Hit
GTTCGTAGGATCTCAACCGTGGTCCCTCCGCCGAAAGTGAGCGGGGAACT17850.1348549912212045No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17780.13432614811837623No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT17570.1327396188098915No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTACAGCCTGAAGATTTTG17380.13130418753078624No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17010.12850887398726546No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCC16520.12480697226746769No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC16380.1237492860618112No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16380.1237492860618112No Hit
GCTAATACCCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCTACAG15920.12027403138608266No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15870.11989628631263391No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15740.11891414912166716No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA15740.11891414912166716No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15580.11770536488663115No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC15510.1171765217838029No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15440.11664767868097463No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15410.1164210316369054No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15160.11453230626966163No Hit
AGTCAGGGTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATG14900.11256803188772811No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14900.11256803188772811No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14880.11241693385834862No Hit
GTCTCCATCTTCCGTGTCTGCGTCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAG14660.1107548555351741No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC14630.11052820849110487No Hit
GGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATC14630.11052820849110487No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14580.1101504634176561No Hit
GAGTTCCCCGCTCACTTTCGGCGGAGGGACCACGGTTGAGATCCTACGAACTGTGGCTGCACCATCTGTCTTCAT14460.1092438752413791No Hit
GTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGTAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCA14390.10871503213855085No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14380.1086394831238611No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA14270.10780844396227383No Hit
GATCAGGAGGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAACC14260.10773289494758409No Hit
GCCCCTAACCTCCTGATCTATGCTGCACCCGGTCTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGAT14220.10743069888882509No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA14150.10690185578599684No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT13900.10501313041875308No Hit
ATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTACAGCCTGAAGATT13880.10486203238937357No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC13500.10199116983116306No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13460.10168897377240407No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13460.10168897377240407No Hit
GGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCTAACCAGCTGCTA13460.10168897377240407No Hit
GTATTAGCAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT13430.10146232672833481No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCC13410.1013112286989553No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT13340.10078238559612705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCT451.943501E-645.71331831
GTCGTAT404.9477887E-543.20530339
ACGCGTA3800.042.32164427
TCGTATG3700.042.1068340
CGCGTAG3900.041.25365428
GACGCGT3900.041.2239926
GTATCAA37800.040.9307671
GCGTAGT3850.040.0293329
CGTATGC3950.039.4932741
CGAGACG4200.038.2403523
AGACGCG4250.037.81476225
ACTACGC655.749498E-736.90270230
TATCAAC42200.036.2529532
ACGAGAC4400.035.72010422
CCCACGA4450.035.3094119
TATACAC5100.034.8185733
CTTATAC5200.034.810961
ATGCCGT4400.034.78638544
ATCAACG44000.034.7698753
CGTAGTA4450.034.65181430
Produced by FastQC (version 0.11.5)