FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970094
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTTACAGTACCCCGGGCACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCAT12080.12452401519852715No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT11920.1228746904939109No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11300.11648355726352291No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10630.10957701006294235No Hit
GGGTACTGTAACTCTGTTGACAGTAGTAAGTTCCAAAATCTTCAGGTTGTAGACTGCCGATGGTGAGAGTGAAAT10600.10926776168082682No Hit
ACAGTACCCCGGGCACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGA10410.10730918859409501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1700.044.7792840
CTCGTAT1950.038.9998139
GTATCAA21200.036.413411
GGTATCA9850.035.8769651
GTGGTAT4350.035.492611
TAATCGC553.0046733E-431.79720152
CTTATAC2300.031.3260881
TATCAAC25050.030.9395432
CGTATGC2650.030.05239541
TCAACGC26200.029.8432944
TTATACA2550.029.5866432
TGGTATC5100.029.5866432
CAACGCA26350.029.543265
ATCAACG26250.029.5251643
AACGCAG26600.029.3945246
TATACAC2850.027.6755453
TATCTCG2750.027.59419436
ACGCAGA29550.026.3426327
AGAGTAC29300.026.0992511
AGCCTAT2800.025.8186932
Produced by FastQC (version 0.11.5)