FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970094
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21500.2216280071828091No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT11810.12174077975948723No Hit
GAGTTACAGTACCCCGGGCACTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCAT11580.11936987549660136No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11280.11627739167544587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22800.036.0905571
GTGGTAT5800.034.8771671
GGTATCA10900.031.769621
TATCAAC25950.031.6998142
TGGTATC6400.031.0621032
ATCAACG28000.029.380453
TCAACGC27950.029.310374
CAACGCA28250.029.1219485
AACGCAG28500.028.9867696
ACGCAGA30200.027.2415567
AGAGTAC30450.026.67879711
TATACTG907.2581697E-626.661415
CGCAGAG31600.025.8176948
CAGAGTA31700.025.73492410
GCAGAGT32600.024.9205939
TGTTAGA3900.024.33265970
AGTACGG31050.022.29834413
GAGTACG31550.022.053612
GTGTATA1206.698751E-520.0001831
GTGTTAG3550.019.82196869
Produced by FastQC (version 0.11.5)