FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences778683
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACAAA300.003094913464.400970
CTTATAC501.1332304E-955.097951
GTAATAC200.007415941551.6476943
TATACAC709.094947E-1249.188283
TTATACA605.7061698E-945.909062
GGTATCA7900.043.1542471
GTATCAA20350.042.304941
GTGGTAT2350.039.5650181
TATCAAC22400.038.735772
TGGTATC2600.037.0803952
TACACAT952.237357E-1036.2445
ATCTACT400.002619483734.436221
TATTGGG553.2940722E-431.3016322
CAGAGTA26850.031.15773810
AGAGTAC27100.030.87030211
CAACGCA28900.030.0235735
AACGCAG28700.029.9928556
GCAGAGT28650.029.9211739
ATGTGTA703.9051833E-529.50917811
ATACACA1302.0190782E-1029.1345984
Produced by FastQC (version 0.11.5)