FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453779
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12790.28185526434674146No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAATAA100.009597505131.0146870
TAAATAG100.009597505131.0146870
TTTTGAC306.3310977E-445.96499329
TGTATAA306.3690566E-445.9092752
GTATCAA13100.042.3357961
GGTATCA4750.042.061771
GTGGTAT1650.039.66641
TATCAAC13950.039.491852
GTGTATA350.001357147539.3681561
TGGTATC1850.037.2237362
ATAAGAG601.3590123E-534.4319573
TAAGAGA601.3590123E-534.4319574
AACGCAG16150.033.9026876
CAACGCA16400.033.3822025
CAGAGTA16550.033.2950310
GCAGAGT16600.033.1910829
AGAGTAC16650.033.0950611
TCAACGC17200.031.8295424
GTATAAG652.3428745E-531.7973581
TATAAGA652.3499479E-531.7833422
Produced by FastQC (version 0.11.5)