FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1117166
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39140.3503507983594202No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21570.19307784160993083No Hit
GTGTTAGCACCTACGTAGCCTGGTACCAACAGACACCTGGCCAGGCTCCC16060.14375661271467266No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATC13450.12039392534323459No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13200.11815612003945698No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12610.11287489952254186No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12190.10911538661219551No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11570.10356562945882707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34700.042.273771
GGTATCA12200.041.104511
TATCAAC38350.038.156242
ATCAACG38900.037.7027553
TCAACGC39000.037.693544
GCGAATT650.00178647737.22847470
CAACGCA39900.036.673985
AACGCAG40850.035.9897086
ACGCAGA43650.033.7592357
CGCAGAG43850.033.6830528
AGAGTAC44800.032.8165111
TGGTATC4350.031.3649372
GCAGAGT47400.031.0164459
CAGAGTA47750.030.86053810
GAGTACG27350.030.18221712
AGTACGG27450.030.07226413
GTGGTAT4850.028.8385961
TTCGCGG500.007448518627.81409642
GCATGCG900.00878494626.88723270
ATAGCGG807.484778E-526.8234363