FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1545382
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTATTCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT61260.3964068430976937No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT57280.370652692991118No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGAAGGCTGCTGATGGTGAGAGTGAATTCTGTC52880.34218076825018023No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC52190.3377158527794422No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG46110.29837282950105537No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC44670.2890547450403848No Hit
GGTCTATAGGGCGTCTAGTCTCCACAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCA44350.28698405960468026No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC44190.285948716886828No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA36280.23476396127300564No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35610.2304284636419992No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33990.2199456186237448No Hit
AGTCTCCACAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC32490.21023928064387962No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC31330.20273304593945057No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31190.20182712106132983No Hit
CTATAGACCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC30320.196197445033008No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29880.19335025255891425No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29670.19199136524173313No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG29050.1879794122100555No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC28960.18739703193126359No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27090.17529646391636503No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26190.16947266112844592No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24320.15737209311354733No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24050.1556249522771716No Hit
GAATTCACTCTCACCATCAGCAGCCTTCAGCCTGATGATTTTGCAACTTA23090.14941289597005789No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22970.14863638893166867No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22470.1454009429383803No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG21620.13990068474979No Hit
GTGGAGACTAGACGCCCTATAGACCAGGAGCTTAGGGGCTTTCCCTGGTT21180.13705349227569621No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC20600.1333003749234817No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20400.13200619652616635No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAGTGTGACAT19960.12915900405207256No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19720.12760598997529413No Hit
CCTTCCACCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCG19480.12605297589851572No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19120.12372345478334806No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18430.11925853931261009No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18350.11874086795368394No Hit
CCACAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAAT18280.11828790551462356No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17510.11330531868495945No Hit
CAGTATAATAGTTATTCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT17270.11175230460818103No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16790.10864627645462417No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16660.10780506049636919No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16580.10728738913744304No Hit
GTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA16470.1065755910189196No Hit
GTTGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGAATATTAATTATTGGTTGGCCTGGTATCAGCAGAAA16440.1063814642593223No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16390.10605791965999345No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16370.10592850182026192No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15620.10107533283032932No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15600.1009459149905978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42750.039.878161
GTGGTAT5850.038.699281
TCGTATG2150.033.79263340
TATCAAC50850.033.5215452
ATCAACG54500.031.4034043
AACGCAG54600.031.0946166
TCAACGC55200.030.9430374
CAACGCA55550.030.686335
TATACAC2650.029.768343
TGTTAGA14450.029.42291670
CGTATGC2500.029.09965941
AGAGTAC58300.028.58802411
CGCAGAG59550.028.391988
CAGAGTA59000.028.3069710
GGTATCA29550.027.9752751
TGACTGG17650.027.68374670
GCAGAGT61550.027.6365669
TTATACA2900.027.2012232
ACGCAGA63150.026.8286087
CTTATAC3000.026.2979181
Produced by FastQC (version 0.11.5)