FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1101162
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAG300.002929999365.3001870
CTTATAC900.057.3334161
TTATACA900.057.3282052
GGTATAT200.00660826253.17732666
TGTAAAA305.765475E-446.85203659
TATACAC1150.044.867593
GTATCAA34250.041.280061
GGTATCA14050.040.8883171
GTGGTAT4100.039.4342081
GTGTAAG350.001367152839.3143421
TTATTCA350.001367458139.3125574
TATCAAC37300.037.901172
TACACAT1700.034.3984875
AGTATCT958.712959E-932.7158334
TGGTATC4900.032.290992
AACGCAG44100.032.0584536
CAACGCA45200.031.2782735
AGAGTAC43900.031.09904311
CAGAGTA43950.031.0636610
TGTATAA1001.493936E-830.95442214
Produced by FastQC (version 0.11.5)