FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1101162
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22150.20115114760589267No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGCT200.007397863551.68100731
TCTATAG200.00748348551.530812
GGTATCA15750.041.24151
GTATCAA38500.040.97371
GTGGTAT5100.040.4328461
TGGTATC5350.039.8119662
TATCAAC41600.037.9874572
TAAGGGT400.002651480534.3523144
AACGCAG48000.032.9254536
AGAGTAC46300.032.27924711
GCAGAGT48050.032.1761639
CAGAGTA46600.032.14517210
CAACGCA49950.031.6400745
ATCAACG51650.030.6610223
AAGTGTA450.00472063430.535394
ACGCAGA51650.030.532167
TCAACGC52100.030.3961944
CGCAGAG51050.030.2853038
ACTATTT500.00702250228.15167260
GTATAAG1057.944582E-726.185081
Produced by FastQC (version 0.11.5)