FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863436
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7750.037.843411
GTATCAA19000.037.7731131
TATCAAC20750.034.4151652
TCAACGC21350.033.6095774
CAACGCA21600.033.380295
TAATCGT1253.6379788E-1233.22998438
ATCAACG21900.032.7655033
AACGCAG22300.032.641886
AGAGTAC23900.030.16446711
ACGCAGA24450.029.6304347
TATAAGA2800.029.5699652
CAGAGTA25150.028.66524110
CGCAGAG25900.027.9699698
TTAATCG1503.274181E-1127.67718137
ACGAACC1606.91216E-1126.1203152
AATAGCG1659.458745E-1125.49124162
AGTACGG21300.024.94098313
GTATTAG2100.024.6459181
GAGTACG21850.024.62893312
AATCGTT1752.2919266E-1023.74674639
Produced by FastQC (version 0.11.5)