FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863436
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11680.1352734887125392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17050.038.4088551
GTGGTAT3400.033.4531631
TATCAAC20100.032.403542
ATCAACG21500.030.4502963
CAACGCA21750.030.2622225
GGTATCA8150.030.0263751
AACGCAG22050.029.850496
TCAACGC22000.029.7582454
TAGTTAG850.005766647429.30719670
ACGCAGA24300.027.0865577
TGTTAGC1155.6979843E-826.9694062
AGAGTAC24150.026.8267111
CAGAGTA25100.026.22323810
CGCAGAG26100.025.2185178
TGGTATC4400.025.0624752
CGATTTG750.001293604524.77162468
TATTAGA1602.230081E-923.6918722
GTATTAG1803.4742698E-1022.9779321
GTTAGCC902.1438254E-422.9712773
AGTACGG20800.022.86348713
Produced by FastQC (version 0.11.5)