FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences965739
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAG150.002366075568.9215851
CTTATAC900.061.2636381
TTATACA900.061.2604642
TATACAC950.058.036233
TTACCAG350.005113469456.733970
TCTTAGC252.5834184E-455.1287088
TACACAT1150.047.942975
GTATCAA28000.043.937521
ATACACA1300.042.411094
GGTATCA9900.041.0744821
TATCAAC30750.040.2301942
AGCCTTA350.001352850839.3980524
GAGCCTA1350.038.3692631
GTGGTAT3200.037.6914941
TCGTATG1600.034.6690740
ATAACTC400.002569137434.573634
TGGTATC3600.034.459012
TAAACAG400.002611308634.4590076
CAGAGTA35450.034.2089110
AGAGTAC35550.033.91886511
Produced by FastQC (version 0.11.5)