FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences965739
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15210.1574959694078835No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTAT150.002210137770.11668460
CCTTAGA150.002371423268.882362
TTAGACT150.002371423268.882364
TAGTTAA200.006775962752.84094265
GATATAA200.00737820251.7153325
CCTATAT200.00740848951.6617743
CCCTAAG306.364239E-445.9215742
GTATAAG851.8189894E-1244.5778661
GTATCAA29050.044.110541
TATAAGA901.8189894E-1242.0947762
AGTTAAT350.001202128540.35593466
TATCAAC31600.040.2177122
TAAGAGA955.456968E-1239.8792654
GAGCATA350.001358025239.3674661
GCCTTAG451.0106345E-438.2739261
TAGACTG451.0109428E-438.2719425
GTGGTAT4200.037.7271581
GACACTT700.001696685337.6200170
GGTATCA10550.036.8953361
ATAAGAG1051.6370905E-1136.081243
Produced by FastQC (version 0.11.5)