FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1423433
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC88970.6250381999012247No Hit
GTATAGGAGTTACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT84040.5904036227908163No Hit
GTCTATTACTGTGCGAGACTTAGTCGGGGTGGCTCGGTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTC54260.3811911062902153No Hit
GTAATAGACAGTCGTGTCTGTGGCGGTCACAGACGTCATCTTCAGGGAGA48450.34037429229194494No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42600.2992764675260444No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37120.2607779923607223No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT36520.25656283084627096No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT36090.2535419650942475No Hit
CTCTAAACTAGACGCCTCATACATGATGAGGGTAGGGGGTTTCCCTGGTT33800.23745409864742492No Hit
AGTTTAGAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGAATCTGGGACAGAATTCACTCTCACCATCAGCAGC33550.23569778134973685No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC33080.23239590483008335No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32430.22782947985609436No Hit
GTCTCTGGTGTCTCCATCAGCAATACTGATTACTTCTGGGGCTGGATCCG32380.22747821639655677No Hit
GGGTAGGGGGTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACCA32110.22558139371505365No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31800.22340356026592048No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC29280.20569988190522492No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28600.2009226988555134No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC28570.20071194077979082No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28020.1968480427248771No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT26500.18616963355493374No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26220.18420255818152312No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26170.18385129472198553No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG25260.177458299758401No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25150.17668552014741826No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24660.17324313824394968No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22800.16017613754915055No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG22600.1587710837110001No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22400.15736602987284964No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGA22160.15567996526706912No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA21920.1539939006612886No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21380.1502002552982824No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20890.1467578733948138No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20850.1464768626271837No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGACTATTGGTAGCTGGTTGGCCTGGTATCAGCAGAAA20590.14465029263758816No Hit
CCCCCTACCCTCATCATGTATGAGGCGTCTAGTTTAGAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGAAT20480.1438775130266054No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT20300.14261296457227002No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT20210.1419806903451023No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG20200.14191043765319478No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC20140.14148892150174963No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19450.13664148576013063No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19120.13432314692718236No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18990.1334098619323846No Hit
CCTCATACATGATGAGGGTAGGGGGTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACCAATAGTCTGA18780.13193455540232663No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18530.13017823810463858No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT18420.12940545849365584No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC18120.12729787773643017No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18080.12701686696880007No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17970.12624408735781734No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAGGAGTTAC17870.12554156043874212No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC17590.12357448506533149No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT17580.12350423237342398No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17480.12280170545434874No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC17300.12153715700001334No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA17090.12006185046995538No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT17000.11942957624278769No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16810.11809477509654477No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT16800.11802452240463723No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT16740.1176030062531921No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16690.1172517427936545No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA16510.1159871943393191No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16460.11563593087978148No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16410.11528466742024387No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG16370.11500365665261379No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16040.11268531781966555No Hit
CTATTATACTGGCAGCTCCTACTACAATCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAAGAA15730.11050748437053237No Hit
CAGTATAGGAGTTACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT15710.11036697898671732No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTTTCCCTCCACC15340.107767629386139No Hit
TGTATGAGGCGTCTAGTTTAGAGAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGAATCTGGGACAGAATTCACT15280.10734611323469387No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG14960.10509802709365317No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA14680.10313095172024254No Hit
GAGTGAATTCTGTCCCAGATTCACTGCCGCTGAACCTTGATGGGACCCCA14570.10235817210925979No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14460.10158539249827704No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA14440.101444887114462No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG14280.10032084404394165No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG14260.10018033866012661No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAACA350.0051443756.6502570
TACGGAT14650.045.56510570
GTGGTAT3200.040.7438281
GTATCAA21450.039.9890371
TATCAAC24600.034.7180372
AACGCAG25500.033.62726
ATCAACG25800.033.235023
CAACGCA26050.032.9160655
TCAACGC26150.032.6578834
TTCTGTC25600.031.82293769
ATCAGGC21200.031.71865836
CTACGGA15250.031.55425369
AGAGTAC27050.031.32321511
CGCAGAG27600.031.070798
CAGAGTA27650.030.6424310
AGGGACC23900.030.50632331
GTAACGT450.004757408530.487593
GCAGAGT28200.030.4097129
AATCATC22450.030.04570432
ACTTTCG24600.030.00376920
Produced by FastQC (version 0.11.5)