FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1824187
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT63990.3507864051218433No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTTCGAAGATT47160.2585261269814992No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42790.23457024965094037No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACATTA41550.22777270093471777No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT40440.22168779845487332No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39240.21510952550369014No Hit
GTCCCAGACCCACTGCCACTGAATTTGGCTGGGATACCAGTGGCCCTGGG37630.20628367596085267No Hit
CTGGTATCCCAGCCAAATTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT37180.20381682360415898No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36900.20228189324888293No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36140.19811565371313358No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAAAGTC35520.19471687935502227No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35350.19378495735360465No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTTCGAAGATTCTGCAGTTTATTACTGTCATCAATATGATAACTGG35100.19241448382210816No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC34670.19005726934793418No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC30820.16895197696288813No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT30710.16834896860902965No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT30340.16632066778241486No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29990.16440200483831977No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29870.16374417754320142No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT29710.16286707448304366No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGGAATAACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT29210.16012612742005067No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28220.15469905223532454No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26900.14746295198902307No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25960.1423099715105962No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGGAATAACTTAGCCTGGTA24270.1330455704376799No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22800.12498718607248051No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22710.12449381560114177No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA22540.12356189359972415No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAATTTGGCTGGGATACCA22200.12169804959688892No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA21630.1185733699450769No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC21360.11709325853106067No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21030.11528423346948531No Hit
GGATACCAGTGGCCCTGGGGGAGGCACCAAAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGG20790.11396857887924867No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC20620.11303665687783106No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20490.11232401064145288No Hit
ATATGATAACTGGCCTCAGACTTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT20450.11210473487641344No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20290.11122763181625568No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG19790.10848668475326269No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19750.10826740898822326No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19710.10804813322318381No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19680.10788367639940422No Hit
GTTTATTACTGTCATCAATATGATAACTGGCCTCAGACTTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA19180.10514272933641122No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA19090.10464935886507248No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18770.10289515274475697No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18560.10174395497829992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15050.040.817851
GTATCAA37750.039.1826551
GTGGTAT5850.038.1321261
TATACCG501.9227935E-434.3113945
TATCAAC44100.033.6111562
ATCAACG46000.031.9973473
TCAACGC46900.031.528774
CAACGCA47400.031.1987535
AACGCAG47950.030.9857066
CGCAGAG50350.029.4405828
AGAGTAC50600.028.9560611
GCAGAGT52800.028.0095089
TATAAGA4550.027.9015712
ACGCAGA54050.027.4252227
CAGAGTA54900.026.68809910
GTAGTTT4450.026.34495470
ATAAGAG4950.024.9523723
TTCGGGG11900.024.08189670
AGTACGG44150.023.70452513
GAGTACT17750.023.19770412
Produced by FastQC (version 0.11.5)