FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875438
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGAC252.641293E-454.881423
TGCTAAA200.00720916352.01891741
CTAATAT306.3160196E-445.99199734
TGGTATC2900.043.76072
GGTATCA9350.043.288821
GTATCAA24850.042.375741
GTGGTAT3100.039.8332861
GCTAATA350.001354515139.38776833
TATCAAC28150.037.6496242
CTTATAC1250.035.6729241
TAAAGAT400.002519381234.71135342
CTCTTAT400.002670731934.3008841
TTATACA1300.034.2989232
AGGGGGG1756.0499588E-933.952370
CGCTAAT1350.033.15739432
AGAGTAC31150.032.69861211
AACGCAG32900.032.52856
CAGAGTA31450.032.386710
ATAGGAC851.2708188E-732.2831883
CAACGCA33300.032.034765
Produced by FastQC (version 0.11.5)