FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1476535
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29020.19654122658792375No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18970.1284764668632982No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15600.10565276136359789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21150.041.483321
GTGGTAT6700.040.922211
GTATCAA49950.039.1782041
TGGTATC7350.037.288082
TATCAAC57650.033.872162
AACGCAG61050.031.9307176
CAACGCA62600.031.0295945
ATCAACG63350.030.7152753
TCAACGC63250.030.6555164
AGAGTAC63850.029.67397511
CAGAGTA64950.029.27691810
CGCAGAG66550.029.1908328
ACGCAGA67400.029.0260337
GCAGAGT74550.026.3800589
TGTTAGA7700.025.71501470
AGTACGG62450.023.536213
GAGTACG63650.023.25395612
ACGTAAC750.002076815322.8381753
GAGTACT18150.022.652512
ATAAGAG4200.022.0225263
Produced by FastQC (version 0.11.5)