FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences765718
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAAT300.00309981564.3750370
CTTATAC600.063.2929341
TTATACA600.063.280532
ACTATTA200.00713208352.15991247
TATACAC800.051.774983
AACGTTG905.2208634E-850.06946670
CTTTATG400.00967622848.28127370
GGTATCA8250.043.5204281
GTATCAA21050.041.0016821
TTAGAAC459.831478E-538.45228638
ATACACA1200.037.393044
TATCAAC23200.037.343452
TCGTATG952.1100277E-1036.45712740
GTGGTAT3250.036.1168061
GTTAGAA501.829372E-434.6002637
TAGGTTA501.8343047E-434.58441534
TACACAT1500.034.516655
TGGTATC3300.033.4706952
AACGCAG26900.032.591936
CAGAGTA26200.031.8776310
Produced by FastQC (version 0.11.5)