FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1526069
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56110.3676766908966764No Hit
ACATTACCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGA48620.3185963413187739No Hit
GGGTAATGTAACTCTGTTGACAGTAGTAAGTTGCAAAGTCTTCAGGCTGCAGACTGGCGATGGTGAGAGTGAACT43280.2836044765996819No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35510.2326893475983065No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34410.22548128557751973No Hit
GAGTTACATTACCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT32950.2159142214408392No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31330.2052987119193169No Hit
GAGTTCACTCTCACCATCGCCAGTCTGCAGCCTGAAGACTTTGCAACTTACTACTGTCAACAGAGTTACATTACC30240.19815617773508276No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29610.1940279240322685No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28550.18708197335769222No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG28460.18649222282871875No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26550.17397640604717088No Hit
GATCTATGCTGCATCCAGTTTGCAGAGTGGTGTCACATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAGTTCA26550.17397640604717088No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC25710.1684720677767519No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25340.16604753782430545No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25210.16519567594912155No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG25200.16513014811256896No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTGACACCTTTTTAAATTGGTATCAGCAGAAA24750.16218139546770166No Hit
GTCTCCATCCTCCCTGTCTGCGTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTGACAC24110.15798761392833482No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT23120.15150035810962678No Hit
ATGTAACTCTGTTGACAGTAGTAAGTTGCAAAGTCTTCAGGCTGCAGACTGGCGATGGTGAGAGTGAACTCTGTC23010.15077955190754808No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22290.1460615476757604No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC21660.14193329397294618No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20380.1335457308942125No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20230.13256281334592343No Hit
ACTTACTACTGTCAACAGAGTTACATTACCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAA20090.1316454236341869No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT20010.13112120094176608No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19810.12981064421071392No Hit
AGTCAGAACATTGACACCTTTTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATG19700.12908983800863524No Hit
GAGTGAACTCTGTCCCAGATCCACTGCCACTGAACCTTGATGTGACACCA19340.12673083589274142No Hit
GTAGTAAGTTGCAAAGTCTTCAGGCTGCAGACTGGCGATGGTGAGAGTGAACTCTGTCCCAGATCCACTGCCA17710.11604979853466653No Hit
ATCTGGGACAGAGTTCACTCTCACCATCGCCAGTCTGCAGCCTGAAGACT17600.11532899233258785No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17420.11414949127464093No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC16960.11113521079322101No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16890.11067651593735277No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16450.10779329112903809No Hit
GTCTTCAGGCTGCAGACTGGCGATGGTGAGAGTGAACTCTGTCCCAGATC16140.10576192819590727No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT15930.10438584362830253No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATT15820.10366503742622385No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGTGACACCACTCTGCAAACT15710.10294423122414517No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC15550.10189578583930346No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15510.10163367449309303No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15330.10045417343514612No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAA350.005684048455.24045670
GTACTTA306.4203754E-445.841516
GTATCAA46050.041.8139571
GTGGTAT5050.040.8528861
TATCAAC52100.036.68962
CTTATAC3400.036.4071351
TACACAT3700.035.3103525
TATACAC3750.034.8395463
TTATACA3800.033.475272
AACGCAG58500.032.735546
CAACGCA59050.032.4306375
ATCAACG60150.031.8947183
TCGTATG2950.031.72428140
TCAACGC60500.031.5965444
TATTCTA1252.830069E-530.93465670
ACACATC4450.030.904396
AGAGTAC60350.030.69854511
CAGAGTA62300.029.73767710
CGCAGAG64100.029.5499538
GACGCTA854.6055007E-528.80620469
Produced by FastQC (version 0.11.5)