FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005759066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1526069
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55600.3643347712324934No Hit
ACATTACCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGA44160.28937092621631133No Hit
GGGTAATGTAACTCTGTTGACAGTAGTAAGTTGCAAAGTCTTCAGGCTGCAGACTGGCGATGGTGAGAGTGAACT43300.28373553227278714No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35520.2327548754348591No Hit
GAGTTACATTACCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT33040.21650397196981264No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32100.2103443553338676No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30100.19723878802334624No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30040.19684562100403064No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29790.19520742509021546No Hit
GAGTTCACTCTCACCATCGCCAGTCTGCAGCCTGAAGACTTTGCAACTTACTACTGTCAACAGAGTTACATTACC29070.19048942085842777No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28650.18773725172321828No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG28100.1841332207128249No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26570.17410746172027608No Hit
GATCTATGCTGCATCCAGTTTGCAGAGTGGTGTCACATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAGTTCA25790.16899629046917275No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC25750.16873417912296232No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24570.16100189440975474No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTGACACCTTTTTAAATTGGTATCAGCAGAAA24050.1575944469090192No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG22720.1488792446475225No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22400.14678235387783906No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22370.14658577036818127No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC22340.14638918685852342No Hit
GTCTCCATCCTCCCTGTCTGCGTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAACATTGACAC22310.1461926033488656No Hit
ATGTAACTCTGTTGACAGTAGTAAGTTGCAAAGTCTTCAGGCTGCAGACTGGCGATGGTGAGAGTGAACTCTGTC21620.14167118262673575No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT20550.13465970411560682No Hit
GAGTGAACTCTGTCCCAGATCCACTGCCACTGAACCTTGATGTGACACCA19790.12967958853760872No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19470.1275826977679253No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19440.12738611425826749No Hit
AGTCAGAACATTGACACCTTTTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATG19070.12496158430582104No Hit
GTAGTAAGTTGCAAAGTCTTCAGGCTGCAGACTGGCGATGGTGAGAGTGAACTCTGTCCCAGATCCACTGCCA18370.12037463574713857No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18160.11899855117953383No Hit
ACTTACTACTGTCAACAGAGTTACATTACCCCGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAA17830.11683613257329781No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17560.11506688098637742No Hit
GTCCCAGATCCACTGCCACTGAACCTTGATGTGACACCACTCTGCAAACT17480.11454265829395657No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17470.11447713045740396No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17360.11375632425532527No Hit
ATCTGGGACAGAGTTCACTCTCACCATCGCCAGTCTGCAGCCTGAAGACT17130.11224918401461534No Hit
GTCTTCAGGCTGCAGACTGGCGATGGTGAGAGTGAACTCTGTCCCAGATC16410.10753117978282764No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16100.10549981684969682No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15800.10353398175311863No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15780.10340292608001342No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15740.10314081473380299No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC15480.10143709098343523No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15470.10137156314688262No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15320.1003886455985935No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)