FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005759067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1518311
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38860.255942293772488No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGAAGGCTGCTGATGGTGAGAGTGAATTCTGTC38840.25581056845402556No Hit
GTATAATAGTTATTCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT37050.2440211524516387No Hit
GGTCTATAGGGCGTCTAGTCTCCACAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCA33900.22327441479380708No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31720.20891635508140297No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29550.19462415802823005No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25940.1708477380457627No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25710.16933289688344483No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA25190.16590803860342182No Hit
AGTCTCCACAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC24230.15958522331722552No Hit
CTATAGACCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC23970.157872794177214No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23320.1535917213271853No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22230.14641269147098324No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC21920.14437094903481568No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20670.13613811663091424No Hit
GTAGTAAACGGCCGTGTCCGCGGCGGTCACAGAGCTCAGTTTCAGGGAGA20620.13580880333475814No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20580.1355453526978333No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20550.13534776472013968No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18810.12388766201390888No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18730.1233607607400592No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18030.11875037459387439No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17520.1153913789730826No Hit
GTGGAGACTAGACGCCCTATAGACCAGGAGCTTAGGGGCTTTCCCTGGTT17440.11486447769923291No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16860.11104444346382264No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16730.11018822889381688No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15980.10524852945147602No Hit
GAATTCACTCTCACCATCAGCAGCCTTCAGCCTGATGATTTTGCAACTTA15830.10426058956300784No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15540.10235057244530271No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15370.10123090723837211No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)