FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697718
Sequences flagged as poor quality0
Sequence length20-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9010.12913526668367448No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAA200.007326309651.80619434
GTATTAC200.00744798751.590831
GTCCTAG200.00744798751.590831
ATTTATG200.00745009851.58713514
TCCTAGA352.3156821E-549.127082
GTCCTAA405.0917097E-542.992361
GTGGTAT3750.039.438321
GTATCAA20650.039.140741
GGTATCA8800.038.6931231
TATCAAC22450.035.844172
ATGCTAG501.8946573E-434.3938871
GTATCAC400.002635143734.3938871
GTACGAT400.002635143734.3938871
CCTCTAG400.002635143734.3938871
TAAACTA905.342372E-934.3938835
TGGTATC4500.034.3889542
GTGTATA652.3695966E-531.7482031
GTTACTA450.00440751530.96517848
TTAAACT1001.4933903E-830.952284
AGTTACT450.00442633530.93822747
Produced by FastQC (version 0.11.5)