FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760002
Sequences flagged as poor quality0
Sequence length20-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15580.20499946052773546No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14750.19407843663569305No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13570.17855216170483762No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT11970.1574995855274065No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11890.15644695671853495No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11350.14934171225865195No Hit
GTATAATAACTGGCTACTTACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT10950.14407856821429418No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA9720.127894400277894No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9350.12302599203686305No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8460.11131549653816701No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8230.10828918871266127No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA8200.10789445290933446No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8080.1063155096960271No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7750.10197341585943195No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACTG200.00754532551.4221154
GGTATCA11600.042.550631
CTTATAC1600.040.703821
GTATCAA28900.040.0885471
TATGGGT350.001390295739.1787534
TATCAAC31900.036.3208582
TTATACA1800.036.183552
TATACAC1900.034.281413
ATACCCG501.931637E-434.281415
AACGCAG35250.032.6767466
CAACGCA35550.032.5938575
TACACAT2000.032.567345
ATACACA2250.031.9959814
ATCAACG36600.031.752453
TCAACGC37100.031.3245224
ATATAGA553.380936E-431.1628672
CGTATGC1900.031.0757341
CAGAGTA37150.030.34959210
TCGTATG1950.030.25269340
AGAGTAC37200.030.21667511
Produced by FastQC (version 0.11.5)