FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences760002
Sequences flagged as poor quality0
Sequence length20-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14820.19499948684345567No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14260.1876310851813548No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12500.16447325138618057No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12250.16118378635845695No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT12070.15881537153849595No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11430.1503943410675235No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11080.14578909002871046No Hit
GTATAATAACTGGCTACTTACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT10250.13486806613666807No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA9960.13105228670450866No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8820.116052326178089No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8710.11460496156589062No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA8350.10986813192596862No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8090.10644708829713606No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7990.10513130228604663No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT7840.10315762326941245No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7660.10078920844945145No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTTA200.007257465751.93046638
CGAGTGT350.001329426939.53715537
GTATCAA29700.038.8797071
GGTATCA12500.038.1025731
CGATACT400.002687755534.2558864
TATCAAC33850.034.002892
GTGGTAT5500.031.7729111
TGGTATC5500.031.7645512
AACGCAG37250.031.0852386
GAATTAG450.00477890430.4576911
CAACGCA38550.030.0369685
TCAACGC38950.029.63864
TAAGAGA1401.6370905E-1129.3621924
AGAGTAC38300.029.33856611
ATCAACG39600.029.152113
CAGAGTA39050.028.95054210
CGCAGAG39700.028.9080058
GCAGAGT39500.028.8809159
TAATCGT756.016369E-527.6962538
ACGCAGA43550.026.5097457
Produced by FastQC (version 0.11.5)