FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1364542
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39280.2878621544811373No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34620.25371150173464796No Hit
CTACAAACCCCCTTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT33860.2481418673811433No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28490.20878800359387986No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC28390.2080551569684187No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCGAGTCAGAGCCTCCTG27660.20270537660255233No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26220.1921523851959119No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT26130.19149282323299685No Hit
CTCTACAAACCCCCTTGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT26000.1905401226198974No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25670.1881217287558756No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25640.18790187476823725No Hit
ACCTACTACAACGCGTCCCTCAAGAGTCGAGTCACCATATCTGTAGACACGTCCAAGAACCAGTTCTCCCTGCAA25490.18680260483004554No Hit
GTAGTAGGTGGTCCCATCATAATAGATTCTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCC25230.18489720360384657No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24780.18159939378927142No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24540.17984056188816466No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC24280.1779351606619657No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG23820.17456406618484444No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT23170.16980056311934702No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22750.1667226072924102No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21480.15741545514905367No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21470.15734217048650756No Hit
GGTTTGTAGAGCGTGCATGCAGTAATAAACCCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAAT21240.15565662324794693No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG21240.15565662324794693No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20640.1512595434951801No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATATGGGTT20230.14825487233078938No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20050.14693574840495932No Hit
GTTTGTAGAGCGTGCATGCAGTAATAAACCCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAAT20000.14656932509222875No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTCACACTGAA19750.14473720852857588No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCT19340.1417325373641852No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19220.14085312141363182No Hit
GTAATAAACCCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAATCTGTGCCTGATCCACTGCCA19130.1401935594507168No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18960.13894772018743284No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCATATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA18440.13513691773503492No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA18370.1346239250972121No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17940.13147268460772918No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT17820.1305932686571758No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC17770.13022684534444523No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG17370.12729545884260066No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC17240.1263427582295012No Hit
GAAATACACAGCCGAATCAGCGGCGGTCACAGAGCTCAGTTGCAGGGAGA17090.12524348829130946No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16950.12421750301566385No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16750.12275180976474157No Hit
GCTAATGCTCTGGGTCTCTGAATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC16710.12245867111455712No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16600.12165253982654987No Hit
CAGTAATAAACCCCAACATCTTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAATCTGTGCCTGATCCACTGCCA16330.11967385393780477No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16010.11732874473632912No Hit
GGCCAAAAGTGTACAAGGGGGTTTGTAGAGCGTGCATGCAGTAATAAACCCCAACATCTTCAGCCTCCACTCTG15750.11542334351013013No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15230.11161254105773219No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC15210.11146597173263997No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGAATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC15160.11109954841990938No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGAATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT15110.11073312510717882No Hit
ATCCATTACTATGCAGGAGGCTCTGACTCGACCTGCAGGAGATGGAGGCC15100.1106598404446327No Hit
ATATGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGT14770.10824144658061093No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA14760.1081681619180648No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTCACACTGAAAATCAGCAGAG14730.10794830793042648No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC14700.10772845394278813No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14690.107655169280242No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATATGGGTTCTAATCGGGCC14500.10626276069186584No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14420.10567648339149692No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT14310.10487035210348966No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14230.10428407480312074No Hit
GTGTATTTCTGTGCGGGAATAATAGTTGCTCCAGATTACTACTTCTACAA14090.10325808952747514No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13860.10157254228891452No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13810.10120611897618395No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGAATCCAGTGGGGATATTGTGATGA13800.10113283431363784No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG13720.10054655701326892No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT200.007531463651.4481838
ACTCGTA1050.041.65418659
TCGTATA1003.6379788E-1240.37352861
CTTATAC4450.038.4428441
GGTATCA10600.037.973511
CGCTAAT4500.037.6525632
CGTATGC4700.037.5102341
TCGTATG4700.037.41272740
TATACAC4850.036.655533
GTGGTAT3800.036.191421
GCTAATC4450.035.86146533
GTATGCC4950.035.6980342
GTATCAA24000.035.4999581
TCGCTAA4800.035.24186331
CGTATAT1152.1827873E-1135.2360162
CTTGAAA5350.035.17755557
TTATACA5000.034.8852
TAATCTC4600.034.10069735
ACTCGCT4950.034.0543329
CTAATCT4600.034.02083234
Produced by FastQC (version 0.11.5)