FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1231792
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20710.1681290347721044No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAAGT350.005310688656.19581270
CTCTAGG200.007444920551.5985642
TACAGTG552.6666385E-950.032945
TATACCC405.0907074E-542.9970555
GTATCAA47700.041.7615931
TGTATAA856.91216E-1140.469462
TCCTAAC350.00136741339.313192
GTGTATA1150.038.892191
GGTATCA18300.038.7287061
TATCAAC52000.038.4343152
CTAAGAG450.00223913435.5566569
GACCTAA400.00224709535.53130367
ATAGGAC601.370224E-534.3976443
AACGCAG60700.032.924276
AGAGTAC59200.032.59906411
GTGGTAT6900.032.410161
CAGAGTA59550.032.40746310
ATCAACG62550.031.9504933
GCAGAGT61750.031.8656679
CAACGCA64200.031.290075
Produced by FastQC (version 0.11.5)