FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1410071
Sequences flagged as poor quality0
Sequence length20-76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21200.15034703926256196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43900.039.638051
GGTATCA17150.039.1018031
GTGGTAT6900.038.3767241
TGGTATC7300.036.264892
TATCAAC48750.035.8267672
TATATAC450.004705107330.5565513
CAGAGTA55750.030.40328610
AACGCAG57800.030.3340236
AGAGTAC56000.030.32895311
CAACGCA58450.029.7603655
GCAGAGT59300.029.1629719
ATCAACG60450.028.8884533
ACGCAGA60500.028.8118447
TCAACGC60600.028.6467694
CGCAGAG61600.028.0182848
AACGTAC500.007799192327.55365628
TAACTCG500.00783576727.5272525
GTCTAAC500.00784527227.52041222
TCTAACT809.752909E-525.8003923
GTGTAAT700.00136112524.55785611
Produced by FastQC (version 0.11.5)