FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956792
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22270.23275696285085995No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13360.13963327452570673No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT11320.11831202602028444No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10560.11036881579277419No Hit
CTACAGACTCCTCGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACG9770.10211205779312535No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9730.1016939940969406No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAA306.355742E-445.93396833
TGTTAGA3900.036.03506570
GTATCAA16100.035.599861
GTGGTAT3350.033.808571
GGTATCA8100.033.4733921
TATCAAC18550.030.8947622
TGGTATC4000.029.1696512
ATCAACG20250.028.299653
AACGCAG20550.028.0564356
CAACGCA20600.027.8202845
TCAACGC20600.027.4871064
AGAGTAC21400.026.30056411
CAGAGTA21600.026.05704310
CGCAGAG22400.025.1264328
ACGCAGA22900.024.877557
CTCGCTA1251.3206409E-724.72779723
AGTGTTA3800.024.67531868
GTGTTAG4100.024.29930769
TTAGGGT1001.6289385E-524.0220644
TCGCTAT1352.7741225E-722.89850424
Produced by FastQC (version 0.11.5)