FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1818011
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCCTCAGGGAGA49150.2703503994200255No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42110.23162676133422735No Hit
GTCTATTACTGTGCGAGACACTCACCCCCCTCGAACTACGATTTTTGGAGTGGTTATCATCCCGAGGGTTGGGA38950.21424512832980658No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31930.17563150057947943No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28770.15824986757505866No Hit
GTCTCTGGTGGCTCCATCCCTGTCTCTGGTGCCTCCATCAGCAGTAGTAATTACTACTGGGGCTGGATCCGCCAG27630.1519792784532107No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC27520.15137422160812009No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26460.14554367382815617No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25920.14257339477043868No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25190.13855801752574654No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24310.13371756276502178No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23330.1283270563269419No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCGTG23270.12799702532052887No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23180.12750197881090927No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC23010.12656689095940563No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC22960.1262918651207281No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22860.125741813443373No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21930.12062633284397067No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21300.11716100727663364No Hit
GTAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTT20460.1125405731868509No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19760.1086902114453653No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT19330.1063249892327384No Hit
CTACAAACTCCTCCGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACG19160.10538990138123476No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG19100.1050598703748217No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19060.10483984970387968No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCCCTGTCTCTGGTGCCTCCATCA18880.10384975668464053No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG18280.10054944662050999No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18220.10021941561409693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG2850.042.48167440
CGTATGC3000.041.5441441
GGTATCA15650.038.5118561
CTCGTAT2950.037.48626739
GTATCAA37050.037.1564251
GTGGTAT5900.035.405761
ACGAGAC4050.032.1301522
TGGTATC6400.031.5669292
TGCGGAT17650.031.45573470
TATCAAC43750.031.3853242
TTAACGG1359.094947E-1230.61593435
ATCAACG45750.029.8652383
TCAACGC46050.029.5963134
ACGATTT12150.029.54986438
CAACGCA46150.029.5321835
AACGCAG46600.029.3939726
CGATTTT12150.029.2957939
ATACACA5800.028.9302564
CTTATAC4500.028.9179291
CTACGAT12450.028.79151536
Produced by FastQC (version 0.11.5)