FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005759089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923467
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48200.5219461009435096No Hit
GTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTT38250.41419996599770215No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34940.3783567793976395No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34820.37705732852392126No Hit
CTACAGACTCCTCGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACG34800.3768407533783016No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34650.3752164397861537No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTC31810.3444627691081544No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28440.30796985707123264No Hit
GACTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGAATTTATTACTGCATGCAAGCTCTACAGACT28040.3036383541588384No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27720.30017315182892296No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27660.2995234263920638No Hit
GTCCCTGACAGGTTCACTGGCAGTGGATCCGGCACAGACTTTACACTGAA26640.2884780939654584No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26160.2832802904705853No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25160.27245153318959964No Hit
GTGTAAAGTCTGTGCCGGATCCACTGCCAGTGAACCTGTCAGGGACCCCG25020.2709355071702616No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23970.2595653120252267No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23400.25339292037506483No Hit
GTCTGTAGAGCTTGCATGCAGTAATAAATTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAGT22570.24440505183184671No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21800.23606690872548775No Hit
GTCAGGGACCCCGGAGGCCCGATAAGAAGCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA21380.23151883066747378No Hit
CTCTACAGACTCCTCGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA21000.2274039029006992No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20710.22426356328921337No Hit
CCCCTGGAGATCCGGCCTCCATCTCCTGTAGGTCTAGTCAGAGCCTCCTG20550.22253096212425566No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTCCGAGGAGT20190.2186326095031008No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGACCTACAGGAGATGGAGGCC20180.21852432193029095No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20010.21668343319252342No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19930.2158171326100445No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18780.203364061736911No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18490.20022372212542516No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTTCTTATCGGGCC18380.19903255882451673No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT18350.19870769610608718No Hit
CTGTAGAGCTTGCATGCAGTAATAAATTCCAACATCCTCAGCCTCCACTC18180.1968668073683196No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17950.1943761931936929No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG17570.19026126542691835No Hit
GCTCTACAGACTCCTCGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT17300.1873375009610522No Hit
GAGTCTGTAGAGCTTGCATGCAGTAATAAATTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAGT16500.17867449513626368No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16460.17824134484502424No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGATCCGGCCTCCATCTCCT16390.17748333183535525No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15020.16264793436040487No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15010.162539646787595No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14610.15820814387520074No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14410.15604239241900358No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14310.15495951669090505No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14270.1545263663996656No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC14200.15376835338999661No Hit
GTGGAGGCTGAGGATGTTGGAATTTATTACTGCATGCAAGCTCTACAGAC14200.15376835338999661No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13990.15149431436098962No Hit
GTCTCCACAGCTCCTGATCTATTTGGCTTCTTATCGGGCCTCCGGGGTCCCTGACAGGTTCACTGGCAGTGGATC13800.14943685047760233No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTTCTTATCGGGCCTCCGGGGTCCCTGA13750.14889541261355307No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT13660.14792082445826435No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13460.14575507300206722No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC13220.14315617125463065No Hit
CCTGTAGGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACACCTATTTG13140.1422898706721518No Hit
GAGTGGAGGCTGAGGATGTTGGAATTTATTACTGCATGCAAGCTCTACAGACTCCTCGGACTTTCGGCGGAGGGA13120.14207329552653206No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT13020.14099041979843352No Hit
GCATAGTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGC12750.13806665533256737No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA12630.1367672044588491No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC12580.13622576659479982No Hit
CTCCACAGCTCCTGATCTATTTGGCTTCTTATCGGGCCTCCGGGGTCCCT12530.1356843287307505No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGGTGTATCCATTACTATGC12520.13557604115794067No Hit
CTCCCTGCCCGTCACCCCTGGAGATCCGGCCTCCATCTCCTGTAGGTCTA12490.1352511784395111No Hit
CTCCTGCATAGTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGAT12390.13416830271141253No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12380.13406001513860266No Hit
CACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTTCTTATCGGGC12160.13167768853678583No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12040.13037823766306755No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA11820.1279959110612507No Hit
AGTCTGTAGAGCTTGCATGCAGTAATAAATTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAGT11660.12626330989629297No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11590.125505296886624No Hit
GTTCACTGGCAGTGGATCCGGCACAGACTTTACACTGAAAATCAGCAGAG11570.12528872174100428No Hit
CTCCTCGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC11410.12355612057604658No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTTCTTATCGGGCCTCCGGGGTCCCTGACAGGTT11340.12279810756637757No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11280.12214838212951844No Hit
ACCTTGGTCCCTCCGCCGAAAGTCCGAGGAGTCTGTAGAGCTTGCATGCAGTAATAAATTCCAACATCCTCAGC11270.12204009455670857No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11240.12171523183827902No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG11230.12160694426546916No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11160.12084893125580015No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11150.1207406436829903No Hit
GTAATAAATTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAA11090.12009091824613115No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11030.11944119280927201No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10920.11825002950836358No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10880.11781687921712417No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT10870.1177085916443143No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10830.11727544135307487No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTC10710.1159759904793566No Hit
TTATTACTGCATGCAAGCTCTACAGACTCCTCGGACTTTCGGCGGAGGGA10700.11586770290654674No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10650.11532626504249746No Hit
GTCTGTGCCGGATCCACTGCCAGTGAACCTGTCAGGGACCCCGGAGGCCCGATAAGAAGCCAAATAGATCAGGAG10620.11500140232406789No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10600.11478482717844818No Hit
GGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTTCTTAT10530.11402681416877918No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10460.11326880115911017No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10300.11153619999415249No Hit
CAGTAATAAATTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGT10290.11142791242134262No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10270.1112113372757229No Hit
CCTTGGTCCCTCCGCCGAAAGTCCGAGGAGTCTGTAGAGCTTGCATGCAGTAATAAATTCCAACATCCTCAGCCT10210.11056161183886376No Hit
GTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAGGTGTATCCA10190.11034503669324405No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10100.10937044853795534No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10020.10850414795547647No Hit
GCCTCCACTCTGCTGATTTTCAGTGTAAAGTCTGTGCCGGATCCACTGCC9890.10709640950894833No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC9870.10687983436332862No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG9750.10558038348961035No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9730.10536380834399064No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9700.10503894562556107No Hit
AGTCAGAGCCTCCTGCATAGTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAG9570.10363120717903293No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9540.10330634446060336No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9480.10265661902374422No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT9330.10103230543159636No Hit
CTCCTGTAGGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACACCTATT9300.10070744271316678No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT9280.10049086756754708No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)