FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923467
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47790.5175063104583054No Hit
GTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTT35360.38290485745565356No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC33830.36633685881574546No Hit
CTACAGACTCCTCGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACG32280.3495522850302176No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTC32150.34814454658368954No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31770.34402961881691496No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30910.33471688755526724No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29450.3189069019250282No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28660.3103521836730495No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC27690.2998482891104934No Hit
GACTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGAATTTATTACTGCATGCAAGCTCTACAGACT27580.298657125809585No Hit
GTCCCTGACAGGTTCACTGGCAGTGGATCCGGCACAGACTTTACACTGAA25740.27873221241257135No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25630.2775410491116629No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25580.27699961124761363No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25440.27548358522827565No Hit
GTGTAAAGTCTGTGCCGGATCCACTGCCAGTGAACCTGTCAGGGACCCCG25310.27407584678174746No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23600.25555867183126196No Hit
GTCTGTAGAGCTTGCATGCAGTAATAAATTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAGT23310.2524183322197761No Hit
CTCTACAGACTCCTCGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA20970.22707904018226965No Hit
GTCAGGGACCCCGGAGGCCCGATAAGAAGCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA20740.22458842600764292No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19780.21419281901789666No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19660.2128933681441784No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19300.20899501552302355No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19070.20650440134839684No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTCCGAGGAGT19040.20617953862996727No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18840.20401378717377017No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18510.20044029727104487No Hit
CCCCTGGAGATCCGGCCTCCATCTCCTGTAGGTCTAGTCAGAGCCTCCTG18170.19675851979550976No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTTCTTATCGGGCC17730.19199386659187603No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17720.1918855790190662No Hit
ATCCATTACTATGCAGGAGGCTCTGACTAGACCTACAGGAGATGGAGGCC17690.19156071630063662No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17550.19004469028129864No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17500.18950325241724936No Hit
CTGTAGAGCTTGCATGCAGTAATAAATTCCAACATCCTCAGCCTCCACTC17330.18766236367948178No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT17310.18744578853386207No Hit
GCTCTACAGACTCCTCGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT16760.18148997202931993No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16510.17878278270907352No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGATCCGGCCTCCATCTCCT16210.17553415552477783No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15910.17228552834048214No Hit
GAGTCTGTAGAGCTTGCATGCAGTAATAAATTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAGT15770.17076950232114413No Hit
GTGGAGGCTGAGGATGTTGGAATTTATTACTGCATGCAAGCTCTACAGAC15730.1703363520299047No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG15530.16817060057370756No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15320.16589656154470056No Hit
GTCTCCACAGCTCCTGATCTATTTGGCTTCTTATCGGGCCTCCGGGGTCCCTGACAGGTTCACTGGCAGTGGATC14800.16026560775858803No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14570.1577749935839613No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14520.15723355571991202No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14190.15366006581718678No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14150.15322691552594733No Hit
GAGTGGAGGCTGAGGATGTTGGAATTTATTACTGCATGCAAGCTCTACAGACTCCTCGGACTTTCGGCGGAGGGA13590.14716281144859536No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC13430.14543021028363765No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13380.14488877241958836No Hit
CTCCCTGCCCGTCACCCCTGGAGATCCGGCCTCCATCTCCTGTAGGTCTA13250.14348103397306022No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTTCTTATCGGGCCTCCGGGGTCCCTGA13190.14283130853620107No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12950.1402324067887645No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC12900.13969096892471525No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC12530.1356843287307505No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12510.1354677535851308No Hit
GTTCACTGGCAGTGGATCCGGCACAGACTTTACACTGAAAATCAGCAGAG12500.13535946601232096No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12460.13492631572108155No Hit
CTCCTCGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC12300.13319371455612383No Hit
CTCCACAGCTCCTGATCTATTTGGCTTCTTATCGGGCCTCCGGGGTCCCT12260.1327605642648844No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT12190.1320025512552154No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12160.13167768853678583No Hit
CTCCTGCATAGTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGAT12090.13091967552711684No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12080.13081138795430697No Hit
GCATAGTAATGGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGC12040.13037823766306755No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA12010.13005337494463798No Hit
CCTGTAGGTCTAGTCAGAGCCTCCTGCATAGTAATGGATACACCTATTTG11940.12929536193496896No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11400.12344783300323671No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT11310.122473244847948No Hit
CACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTTCTTATCGGGC11260.12193180698389873No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT11250.12182351941108886No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA11240.12171523183827902No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG11100.12019920581894102No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11060.11976605552770159No Hit
GTCTGTGCCGGATCCACTGCCAGTGAACCTGTCAGGGACCCCGGAGGCCCGATAAGAAGCCAAATAGATCAGGAG10820.11716715378026503No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGGTGTATCCATTACTATGC10810.11705886620745516No Hit
AGTCTGTAGAGCTTGCATGCAGTAATAAATTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAGT10800.11695057863464532No Hit
ACCTTGGTCCCTCCGCCGAAAGTCCGAGGAGTCTGTAGAGCTTGCATGCAGTAATAAATTCCAACATCCTCAGC10730.11619256562497632No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10650.11532626504249746No Hit
GCCTCCACTCTGCTGATTTTCAGTGTAAAGTCTGTGCCGGATCCACTGCC10620.11500140232406789No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTTCTTATCGGGCCTCCGGGGTCCCTGACAGGTT10610.11489311475125802No Hit
GTAATAAATTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAA10290.11142791242134262No Hit
CCTTGGTCCCTCCGCCGAAAGTCCGAGGAGTCTGTAGAGCTTGCATGCAGTAATAAATTCCAACATCCTCAGCCT10270.1112113372757229No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10180.11023674912043419No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10120.10958702368357505No Hit
CAGTAATAAATTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGT10090.10926216096514547No Hit
GTCCGAGGAGTCTGTAGAGCTTGCATGCAGTAATAAATTCCAACATCCTC10090.10926216096514547No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT9940.10763784737299763No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT9930.10752955980018776No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9810.10623010892646949No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9760.1056886710624202No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9700.10503894562556107No Hit
GGATACACCTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGCTTCTTAT9680.10482237047994135No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9640.10438922018870193No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9630.10428093261589207No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT9570.10363120717903293No Hit
GTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAGGTGTATCCA9430.10211518115969492No Hit
GCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTCCGAGG9410.10189860601407522No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTC9340.10114059300440621No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9320.1009240178587865No Hit
TTATTACTGCATGCAAGCTCTACAGACTCCTCGGACTTTCGGCGGAGGGA9260.10027429242192737No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTAA350.001438641138.90809216
GTATCAA27300.037.0314981
GTATAGG400.002773054634.039051
GCCGTTA400.002773054634.0390515
TATCAAC30950.032.658982
GGTATCA11700.032.002531
AACGCAG31800.031.8947736
ATCAACG32350.031.4509143
TCAACGC33150.030.7945634
CAACGCA33450.030.3182055
GCTTAGG450.00493653630.2569331
CGCAGAG34550.029.3561178
AGAGTAC34500.029.20135511
CAGAGTA34850.029.10341310
ACGCAGA35300.028.7324057
TAGGCAA3300.028.3036870
GTTAAAC500.00823909927.24452218
CGTTAAA500.008243456527.2415717
TTAGGGT500.0082652727.2268182
CGAGGGT808.398638E-426.67569
Produced by FastQC (version 0.11.5)