EGAF00005759091 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005759091
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1872775
Sequences flagged as poor quality	0
Sequence length	20-76
%GC	57

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT	4079	0.21780512875278663	No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT	3869	0.20659182229579098	No Hit
GTACAAACTGGATGCACCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTT	3845	0.20531030155784866	No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC	3781	0.20189291292333572	No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC	3533	0.188650531964598	No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC	3498	0.18678164755509874	No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT	3394	0.18122839102401517	No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC	3079	0.1644084313385217	No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC	2847	0.15202039753841226	No Hit
GTCTCTGGCGGCTCCATCAGTGGTTACTACTGGAGTTGGATCCGGCAGTC	2794	0.1491903725754562	No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG	2788	0.14886999239097062	No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT	2621	0.13995274392278836	No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC	2581	0.13781687602621778	No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG	2570	0.13722951235466085	No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG	2567	0.13706932226241808	No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC	2567	0.13706932226241808	No Hit
CCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC	2560	0.13669554538051823	No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT	2492	0.13306456995634822	No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAACTG	2338	0.12484147855455141	No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC	2336	0.12473468515972289	No Hit
CACCTGCACTGTCTCTGGCGGCTCCATCAGTGGTTACTACTGGAGTTGGATCCGGCAGTCCCCAGGGAAGGGA	2300	0.12281240405280934	No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG	2272	0.12131729652520992	No Hit
GCCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG	2267	0.1210503130381386	No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT	2266	0.12099691634072432	No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG	2226	0.11886104844415374	No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC	2160	0.11533686641481225	No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG	2138	0.11416213907169842	No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA	2128	0.11362817209755577	No Hit
GATCTATGGTGCATCCAGTTTGTACAGTGGGGTCCCATCAAGGTTCAGCGGCAGCGGATCTGGGACAGAATTCA	2104	0.1123466513596134	No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGATTCCAGATGCGACAT	2100	0.11213306456995635	No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC	2098	0.11202627117512783	No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA	2095	0.11186608108288502	No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT	2088	0.11149230420098516	No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT	2081	0.11111852731908532	No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTTTTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA	2019	0.1078079320794009	No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC	1988	0.10615263445955868	No Hit
CAGTTTGTACAGTGGGGTCCCATCAAGGTTCAGCGGCAGCGGATCTGGGACAGAATTCACTCTCACCATCACCAA	1948	0.10401676656298807	No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG	1899	0.10140032838968908	No Hit
ACAATGTCCTCCGCAGTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA	1890	0.10091975811296072	No Hit
GTAATAGATGTACCCAATCCATTCCAGTCCCTTCCCTGGGGACTGCCGGA	1886	0.10070617132330364	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	35	0.001380723	39.236855	5
GTATCAA	2730	0.0	37.982307	1
GTGGTAT	555	0.0	37.737564	1
TCGTATG	205	0.0	35.468918	40
CGCTAAT	195	0.0	35.328598	32
CGTATGC	215	0.0	33.84381	41
TATCAAC	3295	0.0	31.256826	2
GCTAATC	200	0.0	31.027447	33
ATCAACG	3500	0.0	29.721918	3
TCGCTAA	255	0.0	29.700876	31
CTAATCT	210	0.0	29.561842	34
TCAACGC	3560	0.0	29.317425	4
AACGCAG	3595	0.0	28.840996	6
CAACGCA	3640	0.0	28.578764	5
TATACAC	280	0.0	28.20149	3
TTATACA	280	0.0	28.199984	2
ATATCCG	50	0.0072918073	27.93653	51
CTTATAC	295	0.0	27.933558	1
CTAACGA	50	0.007909957	27.474602	23
GTATGCC	295	0.0	27.045568	42

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content