FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1872775
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT40240.21486831039500207No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38930.20787334303373337No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT37890.20232008650264982No Hit
GTACAAACTGGATGCACCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTT36690.19591248281293802No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35030.18704863104217004No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC32940.17588872128258867No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC31400.16766562988079187No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29790.1590687615970952No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29760.1589085715048524No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26920.14374390943920118No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC26120.13947217364606No Hit
GTCTCTGGCGGCTCCATCAGTGGTTACTACTGGAGTTGGATCCGGCAGTC25970.138671223184846No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24690.1318364459158201No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAACTG24650.13162285912616303No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24380.13018114829597788No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24370.13012775159856363No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24330.12991416480890658No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23610.1260696025950795No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG22900.12227843707866667No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22790.12169107340710976No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC22210.11859406495708241No Hit
CCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC22060.11779311449586843No Hit
CACCTGCACTGTCTCTGGCGGCTCCATCAGTGGTTACTACTGGAGTTGGATCCGGCAGTCCCCAGGGAAGGGA21710.11592423008636916No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA21060.11245344475444194No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT21050.11240004805702768No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG20550.10973021318631442No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC20320.10850208914578634No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20300.1083952957509578No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC20080.10722056840784398No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19950.10652641134145853No Hit
GCCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19840.10593904766990161No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19490.10407016326040235No Hit
GATCTATGGTGCATCCAGTTTGTACAGTGGGGTCCCATCAAGGTTCAGCGGCAGCGGATCTGGGACAGAATTCA19420.1036963863785025No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT19140.10220127885090308No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA18910.10097315481037497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31050.037.791571
GTGGTAT6050.037.4299281
TATCAAC36750.032.2032282
ATCAACG39200.030.1022153
CAACGCA39750.029.4291745
AACGCAG39850.029.3561126
TCAACGC40200.029.3541834
AGAGTAC40850.028.2190811
CGCAGAG42300.027.73848
CAGAGTA42150.027.43013610
GCAGAGT43250.026.891149
ACGCAGA47050.024.865117
TGTTAGA9700.023.99523770
AGTACGG43150.023.13704313
GAGTACG43950.022.95007312
TACGGGG19300.021.86586215
GGTATCA27400.021.5381471
ACGGGGG7500.021.5008416
TTCGGGG16000.021.01249770
CGATTAA1853.9727762E-420.96881170
Produced by FastQC (version 0.11.5)