FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1706827
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT79600.4663624374350769No Hit
GTCTACAAACTCCTCGGACTTTCGGCGGAGGGACCAGGGTGGAGATCAAA71410.41837866403566387No Hit
AGCCAGGACAGTCTCCACAGCTCCTAATCTATTTGGGCTCTTATCGGGCC62570.3665866546521704No Hit
GTTTGTAGACCTTGCATGCAGTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAGT58720.3440301799772326No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT48220.2825125217728569No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC48160.2821609922974033No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47540.2785285210510497No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG44960.2634127536065459No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44180.25884287042564946No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC43780.2564993405892923No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCCCTCCGCCGAAAGTC42180.24712522124386363No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT40600.2378682783902528No Hit
GGCACAGACTTCACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGGTCTA39840.23341557170117416No Hit
GGTCTAGTCAGAGCCTCCTGCAGAGTCTTGGAAAGAACTATTTGGATTGGTACCTGCAGAAGCCAGGACAGTCTC39620.2321266302911777No Hit
GTCCTGGCTTCTGCAGGTACCAATCCAAATAGTTCTTTCCAAGACTCTGC39190.2296073357170938No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT38200.22380709937210977No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGACTTCACACTGAA35520.20810544946851672No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC34680.20318403681216668No Hit
GTCTTGGAAAGAACTATTTGGATTGGTACCTGCAGAAGCCAGGACAGTCTCCACAGCTCCTAATCTATTTGGGCT34370.20136780118898984No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32730.19175932885992547No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32540.19064615218765582No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT32300.18924003428584152No Hit
GTCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCGGAGGCCCGATAAGAGCCCAAATAGATTAGGAG31740.18595909251494147No Hit
GTCAGGGACCCCGGAGGCCCGATAAGAGCCCAAATAGATTAGGAGCTGTGGAGACTGTCCTGGCTTCTGCAGGTA27140.159008499396834No Hit
GTTCAGTGGCAGTGGATCAGGCACAGACTTCACACTGAAAATCAGCAGAG27130.15894991115092508No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG27000.158188263954109No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG27000.158188263954109No Hit
CTCCTGCAGAGTCTTGGAAAGAACTATTTGGATTGGTACCTGCAGAAGCCAGGACAGTCTCCACAGCTCCTAAT26990.15812967570820008No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26980.15807108746229115No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA26660.1561962635932054No Hit
GTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAGTCTGTGCCTGATCCACTGCCA26470.15508308692093575No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26130.15309108656003215No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26020.1524466158550339No Hit
CCCCTGGAGAACCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG25510.14945861531367854No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA25380.14869696811686248No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCCTGGTCC25140.14729085021504815No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25040.14670496775595887No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTAATGACTCAGTCTCCAGTCTCC24770.14512308511641778No Hit
GTCTCCACAGCTCCTAATCTATTTGGGCTCTTATCGGGCCTCCGGGGTCC24350.14266237878824273No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTAATGACTCAGTCTCCAGTCTCCCTGCCCGTCACCC24230.1419593198373356No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24100.14119767264051952No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTAATGACTCAGTCTCCAGT23460.13744802490234803No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23180.135807554016898No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA22620.13252661224599796No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22470.13164778855736403No Hit
GTCTCCAGTCTCCCTGCCCGTCACCCCTGGAGAACCGGCCTCCATCTCCT22410.13129625908191048No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22230.13024167065554976No Hit
GACCAGGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT21620.12666778765510506No Hit
GTTTATTACTGCATGCAAGGTCTACAAACTCCTCGGACTTTCGGCGGAGGGACCAGGGTGGAGATCAAACGAA21480.12584755221238006No Hit
GTGGAGACTGTCCTGGCTTCTGCAGGTACCAATCCAAATAGTTCTTTCCA21420.12549602273692648No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21420.12549602273692648No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21300.12479296378601933No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21000.12303531640875144No Hit
CAGTAATAAACTCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTGAAGTCTGTGCCTGATCCACTGCCA20940.12268378693329786No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20720.12139484552330143No Hit
TTCCAAGACTCTGCAGGAGGCTCTGACTAGACCTGCAGGAGATGGAGGCC20600.12069178657239427No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTAATGA20500.12010590411330499No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG20320.11905131568694426No Hit
GTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGGTCTACAAAC19660.11518449145695492No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT18990.11125907898105666No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC18930.11090754950560308No Hit
CTTATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGATCAGGC18770.10997013757106022No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18480.10827107843970128No Hit
ACCCTGGTCCCTCCGCCGAAAGTCCGAGGAGTTTGTAGACCTTGCATGCAGTAATAAACTCCAACATCCTCAGC18450.10809531370197449No Hit
TTATTACTGCATGCAAGGTCTACAAACTCCTCGGACTTTCGGCGGAGGGA18430.10797813721015663No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC17840.1045214307015298No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTAATGACTCAGTCTC17560.10288095981607977No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA17460.10229507735699049No Hit
TCTCCACCCTGGTCCCTCCGCCGAAAGTCCGAGGAGTTTGTAGACCTTGCATGCAGTAATAAACTCCAACATCCT17370.10176778314381012No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17270.10118190068472083No Hit
GACTTCACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGGTCTACAAACT17220.10088895945517617No Hit
GAGTGGAGGCTGAGGATGTTGGAGTTTATTACTGCATGCAAGGTCTACAAACTCCTCGGACTTTCGGCGGAGGGA17180.10065460647154045No Hit
GTCTTGGAAAGAACTATTTGGATTGGTACCTGCAGAAGCCAGGACAGTCTCCACAGCTCCTAATCTATTTGGG17130.10036166524199583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34100.041.3273051
GGTATCA13600.037.4050641
TATCAAC38700.036.405412
TCAACGC40800.034.698024
GTGGTAT5450.034.687561
ATCAACG41100.034.278543
AACGCAG41050.034.2395976
CAACGCA41300.034.196755
CGCAGAG43850.031.4263028
AGAGTAC43650.031.41283611
TGGTATC5850.031.1324332
CAGAGTA43800.031.06988110
ACGCAGA45700.030.6805047
TGTTAGA11950.028.58830570
TATAAGA3750.027.4905282
CTACCGA6300.025.82470169
AGTACGG41550.025.63947713
TATTAGA2150.025.572582
GAGTACG42150.025.35603312
ATTAGAG1801.2732926E-1124.8171083
Produced by FastQC (version 0.11.5)