FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences878676
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAAGT151.8543206E-4130.7874870
TCATAAG200.004351258759.09802669
TTGTAAG200.007349290451.7663229
GTCTAGA200.00737249151.725072
TCTAGAC200.00737249151.725073
CCCGTAA200.00737249151.725072
GTAATTA200.0073758151.71918516
TCTAAAC200.0073774751.7162412
GTGGTAT3350.050.446991
TGGTAAT306.330965E-445.9699914
TGGTATC3900.042.4410862
GGTATCA9350.042.051091
GTATCAA23150.040.523031
GTTAGAT350.001350840839.4095763
TATCAAC26300.035.925652
GGAGCTA501.8632205E-434.49319526
TGGATAG450.004633010430.6518975
CTAGCTC450.004633010430.6518974
AACGCAG31200.030.2834846
AGAGTAC30400.030.2812211
Produced by FastQC (version 0.11.5)