FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1600865
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTATA601.8189894E-1257.182962
AATCCTA200.00752406351.4614511
CTTATAC1700.044.4078251
GTCTTAG405.164055E-542.8939171
TTATACA1750.041.171732
GGTATCA23900.040.4889871
TATACAC1900.039.727113
GTATCAA57800.038.767791
TATCAAC64400.034.8958132
CTCTTAT959.273208E-932.509081
CTAAACA553.3434195E-431.2248426
AACTAAA553.3526254E-431.21020324
TAACTAA553.3556987E-431.20532623
AGGGGGG2601.0913936E-1130.32277970
AACGCAG74250.030.1279136
TCTTAGC802.8841896E-630.0210552
CAGAGTA73050.029.54072810
AGAGTAC73000.029.46696511
GCAGAGT76250.029.200869
ATCAACG77950.029.1383
Produced by FastQC (version 0.11.5)