FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1600865
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23950.14960661892164548No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAAG200.007459628451.5735227
GTTACTC403.4593628E-545.90563668
TGTATAA1100.040.577842
GTATCAA59500.040.3466031
GGTTACT458.1539954E-539.69629367
GGCTTAA350.001378406439.2499121
GTGTATA1150.038.8232841
TATCAAC63950.037.637062
CCATTAG601.385295E-534.3436741
TTAGACT400.002659181134.332954
GGTATCA24700.033.3703731
CAGAGTA71000.032.5941610
AACGCAG74100.032.4816446
AGAGTAC71550.032.29562411
CAACGCA77200.031.309785
GCAGAGT76050.031.2876019
ACCAATA553.350411E-431.2137222
ACGCAGA78650.030.6025437
TGTAATT450.004732859330.52008212
CTGATAA450.004732859330.52008213
Produced by FastQC (version 0.11.5)