FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences946967
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35150.3711850571350427No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28240.2982152493170301No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23320.24625990134819906No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCCGGCCAAAAGTC22280.23527747007023475No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21630.22841345052150708No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21500.22704064661176154No Hit
CTACAAACTCCTCGGACTTTTGGCCGGGGGACCAAGCTGGAGATCAAACG21490.2269350463110119No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21440.22640704480726362No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20060.211834203303811No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18460.19493815518386595No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18060.19071414315387972No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT17850.1884965368381369No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17810.18807413563513828No Hit
GTAATGGACACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTT17550.1853285278156472No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17520.18501172691339826No Hit
GTAATAGACGGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGAAACA17400.18374452330440239No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16850.17793650676317127No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT16000.16896048119945045No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15800.1668484751844573No Hit
GTCTATTACTGTGCGAGAGATATCCTCTTTTACGATTTTTGGACTGGTTACTCGAAGTCACTAGACCACTGGGGC15710.16589807247771043No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15660.16537007097396214No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAACACCAATATCCTCAGCCTCCACTCTGCTGATTTGCAGTGTAAAAT15560.16431406796646558No Hit
GATTTTACACTGCAAATCAGCAGAGTGGAGGCTGAGGATATTGGTGTTTATTACTGCATGCAAGCTCTACAAACT14900.15734444811698825No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14790.156182844808742No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCG14710.15533804240274476No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14440.15248683428250404No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14110.14900202435776538No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13730.14498921292927844No Hit
GTCAGGGACCCCGGAGGCCCGAGTAGAACCCAAATAGATCAGGAGTTGTGGAGACTGCCCTGGCTTCTGCAGGTA13700.14467241202702946No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13680.14446121142553014No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCGGAGCCTCCTG13640.1440388102225315No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG13460.1421380048090377No Hit
GTAATAAACACCAATATCCTCAGCCTCCACTCTGCTGATTTGCAGTGTAAAATCTGTGCCTGATCCACTGCCA13180.13918119638804732No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC13140.1387587951850487No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGCAAATCAGCAGAG12930.1365411888693059No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12890.13611878766630728No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12820.13537958556105967No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12520.13221157653857No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12510.1321059762378203No Hit
GTCTCCACTCTCCCTGCCCGTGACCCCTGGAGAGCCGGCCTCCATCTCCT12370.13062757202732514No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12340.13031077112507616No Hit
CTCCTGCATAGTAATGGACACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAACTCCTGAT12120.12798756450858373No Hit
CAGTAATAAACACCAATATCCTCAGCCTCCACTCTGCTGATTTGCAGTGTAAAATCTGTGCCTGATCCACTGCCA11860.12524195668909263No Hit
AGCCAGGGCAGTCTCCACAACTCCTGATCTATTTGGGTTCTACTCGGGCC11850.125136356388343No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11680.12334115127559883No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCTCGGACTTTTGGCCGGGGGA11520.12165154646360432No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11450.12091234435835674No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAGATTGTGATGACTCAGTCTCCACTCTCC11330.11964514074936086No Hit
GTTTATTACTGCATGCAAGCTCTACAAACTCCTCGGACTTTTGGCCGGGGGACCAAGCTGGAGATCAAACGAA10970.11584352992237322No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAGATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTGACCC10940.11552672902012426No Hit
CTCCACAACTCCTGATCTATTTGGGTTCTACTCGGGCCTCCGGGGTCCCT10910.11520992811787528No Hit
CTCCCTGCCCGTGACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA10500.11088031578713937No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10430.11014111368189176No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10400.1098243127796428No Hit
GTGGAGGCTGAGGATATTGGTGTTTATTACTGCATGCAAGCTCTACAAAC10360.10940191157664417No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10350.10929631127589451No Hit
CCAATATCCTCAGCCTCCACTCTGCTGATTTGCAGTGTAAAATCTGTGCC10260.1083459085691476No Hit
GGATATTGGTGTTTATTACTGCATGCAAGCTCTACAAACTCCTCGGACTTTTGGCCGGGGGACCAAGCTGGAGAT10210.10781790706539932No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10180.10750110616315035No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10120.10686750435865242No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGAGATTGTGATGACTCAGTCTCCACT10110.10676190405790277No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG10000.10560030074965654No Hit
GGTCTAGTCGGAGCCTCCTGCATAGTAATGGACACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC9970.10528349984740756No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9970.10528349984740756No Hit
GTTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTGTCCATTACTATGCAGGAGGCTCCGA9930.10486109864440893No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA9800.10348829473466341No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9770.10317149383241443No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9680.10222109112566753No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAC200.006979010552.4478153
GGTATCA8400.042.8684431
TAGATTG350.001389974539.1815875
GTATCAA17650.035.7520371
ACGCGTG400.002596518334.4988635
TGGTATC2800.033.0559732
GTGGTAT2900.031.9295981
TCTGGAG8300.031.58209870
TATCAAC20000.031.5378482
TGTTAGC553.3809157E-431.163882
CATTATA450.004767792330.4729614
ATCAACG20450.030.340973
TCAACGC20500.030.2685684
CAACGCA20750.030.0706885
AACGCAG21200.029.270686
CGCAGAG21850.028.3999258
AGAGTAC22200.028.26104411
CAGAGTA22550.027.82240110
GCGTTGT5650.027.50207562
GTCTAGG500.0079627327.4358021
Produced by FastQC (version 0.11.5)