FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences879164
Sequences flagged as poor quality0
Sequence length20-76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATGT100.00975084130.3348570
TAAAAAT150.002213517270.08913458
CTTATAC554.1836756E-1156.3213921
TTATACA554.1836756E-1156.3181882
AAACCTA200.007390795751.69263528
GTGGTAT2850.044.683831
GTGCTAG405.0712304E-543.023291
GTATCAA26500.041.6921
GGTATCA10350.041.5683941
TATCAAC28550.038.81672
TACACAT801.6789272E-938.7187585
GTATAAG1200.037.286851
TATAAGA1200.037.2847252
TGGTATC3550.036.840382
CCTGACA400.002431492834.9635755
TATTTCA400.00247941934.8247247
TAGGAGT400.002626970434.416674
TAAGAGA1300.034.416674
TATCCAA400.002626970434.416675
ATAAGAG1350.033.141983
Produced by FastQC (version 0.11.5)