FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005759105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005759105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1598829
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT49770.31129032560705366No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC48430.3029091916646495No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGAAGATACA47840.2992189908989642No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT44350.27739051518329977No Hit
GTGTATTACTGTGCGAGAGCCGTTTGGTTCGGGGAGTTATGGGACTACTG39830.24911982457160833No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC38880.243177975881098No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT37330.23348338064921262No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG34060.21303091199871907No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30680.19189043981564008No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC29720.18588604534944012No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29390.18382203475168388No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG28890.18069474596720475No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28880.18063220019151516No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT28720.17963146778048183No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC28170.1761914501175548No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA27560.17237615780049023No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTGGCTTTTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAAGGG27230.17031214720273402No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA27130.16968668944583817No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG26290.16443284428791322No Hit
CTCCAGCCCTTTCCCTGGAGCCTGGCGGACCCAGCTCATCCAAAAGCCAC25620.16024227731671117No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA24810.15517606948585494No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24270.15179859759861747No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT23900.14948440389810294No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC23850.14917167501965503No Hit
CAGTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGAAGATA23490.14692002709483No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG23360.14610693201086547No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT23220.14523129115121128No Hit
GGGCTGGAGTGGGTGGCCAACATAAAGCAAGATGGAAGTGAGAAATACTA22630.1415410903855259No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT22250.13916435090932175No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGA22170.1386639847038051No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21920.1371003403115655No Hit
GTATAACAGTGCCCCTCGAACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT21760.1360996079005322No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGACAAGAG21750.13603706212484262No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA21710.13578687902208428No Hit
GTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGATGTCG21620.13522396704087805No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG20980.13122103739674473No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACAAGAAAGTTGAGCCCAAATCTTGTGA19950.1247788225007177No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAG19950.1247788225007177No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC19760.12359045276261564No Hit
GCATAGATCAGGAGCCTAGGACCTTTACCTGGTTTCTGCTGATACCAGGC19760.12359045276261564No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT19700.12321517810847814No Hit
GATCTATGCTGCATCCACTTTGCAATCAGGGGTCCCATCTCGGTTCAGTG19630.12277735767865106No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCACCACCACGCATGTGACCTCAGGGGT19420.12146389638916984No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19380.12121371328641149No Hit
GTATTACTGTGCGAGAGCCGTTTGGTTCGGGGAGTTATGGGACTACTGGGGCCAGGGAATCCTGGTCACCGTCGC19210.12015043509968858No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT19070.11927479424003443No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19010.11889951958589692No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC18820.11771114984779486No Hit
GTTATGGGACTACTGGGGCCAGGGAATCCTGGTCACCGTCGCCTCAGCCTCCACCAAGGGCCCATCGGTCTTCCC18810.11764860407210527No Hit
GCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGGTTTTC18780.11746096674503652No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG18510.11577223080141778No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACAGCACGTACCGTGTGGTCAGCGTC18490.11564713925003862No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC18400.11508422726883237No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18140.11345803710090321No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG18040.1128325793440074No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA18010.11264494201693866No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT17910.11201948426004281No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17850.1116442096059053No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17670.11051838564349283No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTT17630.11026820254073451No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA17460.10920492435401159No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17000.1063278186722908No Hit
CTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGGTCAGGCTGACCTGGTTCTTGGTCAGCTCATC16940.1059525440181533No Hit
CTGTTATACTTTTGACAGTAATAAGTTGCAACGTCTTCAGGCTGCAGGCTGGTGATGGTGAGAATGAAATCTGTC16770.10488926583143038No Hit
TGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACC16650.10413871652315539No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGTGGCTTTTGG16610.10388853342039706No Hit
GTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTA16590.10376344186901788No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTC16470.10301289256074289No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16440.10282525523367414No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGAGTGAGGTGCAGTTGGTGGAGT16410.10263761790660539No Hit
CGGTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTTTCTTGT16360.10232488902815748No Hit
CTCCCACTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGGTCAGGCTGACCTGGTTCTTGGTCAG16120.1008237904116075No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC16060.10044851575747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG2500.042.9142840
CTCGTAT2500.041.49462539
GTATCAA19100.037.391511
GTGGTAT3050.037.1498531
CGTATGC2900.037.01257741
TATACAC3100.036.546093
TTATACA3150.033.7862362
CTTATAC3200.033.262491
TACACAT3700.031.547575
GGAGCTA3550.030.99682629
ATACACA4100.030.981794
TATCAAC23500.030.825012
ACGAGAC3350.030.74337422
TCTGGAG22150.030.37257270
CACGAGA3400.030.29126721
ATCAACG24600.029.7257733
ATGCCGT3650.029.48911744
GTATGCC3650.029.43789742
AGACGGA3500.029.43685225
AACGCAG25400.028.6543676
Produced by FastQC (version 0.11.5)